10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) lessThan i greaterThan Neolentinus lessThan /i greaterThan lessThan i greaterThan kauffmanii lessThan /i greaterThan (A.H. Smith) were found growing on decaying logs of wood in their large numbers within the kothaiyar forest area of Kanyakumari district, Tamilnadu, India. The present study was aimed to investigate the effect of different semi-synthetic growth media, pH, temperature, carbon, nitrogen and amino acids on vegetative growth of lessThan i greaterThan N. kauffmanii lessThan /i greaterThan . The best mycelial extensions were observed on Malt-yeast agar and Potato-dextrose agar respectively. This fungus produced best mycelial biomass (301 mg/ cm lessThan sup greaterThan 3 lessThan /sup greaterThan ) at 25°C after 17 days of incubation while less biomass was produced at 20°C and 40°C after 17 lessThan sup greaterThan th lessThan /sup greaterThan day. Likewise, very good biomass was produced at pH 6.5 after 17 lessThan sup greaterThan th lessThan /sup greaterThan day of incubation. Among the carbohydrate sources tested, fructose stimulated highest biomass yield of 328 mg/cm lessThan sup greaterThan 3 lessThan /sup greaterThan at 1% concentration closely followed by sucrose (307 mg/cm lessThan sup greaterThan 3 lessThan /sup greaterThan ). All the nitrogen compounds investigated enhanced significant biomass production 0.5% of peptone stimulated the best biomass yield (343 mg/cm lessThan sup greaterThan 3 lessThan /sup greaterThan ) followed by beef extract (326 mg/cm lessThan sup greaterThan 3 lessThan /sup greaterThan ) at 0.5% concentration, different amino acid tested glycine (314 mg/cm lessThan sup greaterThan 3 lessThan /sup greaterThan ) supported the maximum growth. The implications of these findings were discussed. JOHNSY, G. AND KAVIYARASAN, V. Sporophores, Neolentinus kauffmanii, submerged culture, biomass, carbon, nitrogen compounds, amino acids. 469-478