International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 1
2013 (January - March)
DIVERSITY OF BIOSURFACTANT-PRODUCING STREPTOMYCES ISOLATES FROM HYDROCARBON-CONTAMINATED SOIL
Actinomycetes are unsurpassed as producers of bioactive metabolites, primarily those with antimicrobial/anticancer properties; however, their capacity for producing other natural products such as biosurfactants, has been less explored. Biosurfactants are heterogeneous surface active compounds and can be potentially deployed for bioremediation of hydrocarbons and as emulsifying agents in agriculture, pharmaceutical and food industries. In this study, lessThan i greaterThan Streptomyces lessThan /i greaterThan was selectively isolated and characterized from hydrocarbon-contaminated soil. The isolates were dereplicated based on morphology and antimicrobial properties and representative isolates screened for biosurfactant production using drop-collapse test, emulsification assay and bacterial adherence to hydrocarbons (BATH) test. lessThan i greaterThan Streptomyces lessThan /i greaterThan sp. PN-18 exhibited high antimicrobial activity, significant cell-surface hydrophobicity ( greaterThan 95%) and emulsification activity ( greaterThan 70 EU mL lessThan sup greaterThan -1 lessThan /sup greaterThan ). The best production medium was found to be tryptone soy broth in which emulsification activity increased to 110 EU mL lessThan sup greaterThan -1 lessThan /sup greaterThan with emulsification index (E lessThan sub greaterThan 24 lessThan /sub greaterThan ) of 65%. Nearly complete 16S rDNA sequence analysis (GenBank accession GQ856644) of PN-18 showed similarity to lessThan i greaterThan S. rochei lessThan /i greaterThan (97.9%), though with significant differences in morphological and physiological characteristics. The study demonstrates lessThan i greaterThan Streptomyces lessThan /i greaterThan diversity in hydrocarbon-impacted environments and the possibility of exploiting PN-18 for bioremediation.
NEHA PANJIAR, REEMA GABRANI, AND INDIRA P.SARETHY
Streptomyces, biosurfactant, hydrocarbon-contaminated soil, bioremediation
524-535