10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) Medicinal plant contains matter that can be used for therapeutic reason or which are precursors for the production of useful drugs. Very few and periodic works are there in the literature regarding the phytochemistry and TLC of lessThan i greaterThan Viola odorata lessThan /i greaterThan L. TLC profiling of plant extract gives an idea about the presence of various phytochemicals. Different Rf (Retention factor) value of various phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals. HPLC profile of petroleum ether extract of lessThan i greaterThan Viola odorata lessThan /i greaterThan have characteristics peaks at retention time 2.885, 3.370, 3.487, 3.900, 4.067, 4.191, 5.207, 6.724 (Stigmasterol), 17.698 (Lupeol), 18.174 (β-Sitosterol), 23.118. These peaks showed that there are different compounds and characteristic fingerprints for each drug to judge in an herbal formulation. SANDHYA MITTAL Viola odorata L., HPLC, TLC Profiling and Retention factor (Rf.). 542-549