10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) Global Warming is an average increase in the temperature of the atmosphere near the Earth's surface and in the Troposphere, which can contribute to changes in global climate patterns. Changing climate may promote harmful physical impacts such as effects on weather and radioactive forcing. Global warming causes the rise in the sea level and ocean temperature. This change affects the ecosystem and lifestyle of living organism. The butterfly (Tawny Coster) was collected from Chennai,chengalpattu and Tanjore, Tamil Nadu .The Genomic DNA was isolated from the samples and the DNA was amplified in the polymerase chain reaction (PCR). Gene specific PCR was used to find the presence of COI gene. RNA levels are determined by reverse transcriptase polymerase chain reaction. The purpose of this research work is helpful to find out how Global Warming is affecting small creatures. If this Global Warming is continuously increasing it leads to extinction of small creatures. DR.S.ANBUSELVI Global warming, Genomic DNA, Butterfly, COI gene 713-718