10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) Genomic DNA isolated from zygotic embryos (seeds) of 7 chilli pepper varieties, namely lessThan i greaterThan Capsicum annuum L., lessThan /i greaterThan California wonder, Yolo wonder, G-5, Pusa sadabahar, Pusa jyothi and LCA-335 and lessThan i greaterThan Capsicum chinense lessThan /i greaterThan lessThan i greaterThan Jacq. lessThan /i greaterThan variety Kotpar, was PCR-amplified using lessThan i greaterThan Pun1 lessThan /i greaterThan allele specific primer lessThan i greaterThan MAP1 lessThan /i greaterThan . A DNA band admeasuring 479 bp was observed in lessThan i greaterThan C annuum lessThan /i greaterThan L lessThan i greaterThan . lessThan /i greaterThan , varieties California wonder and Yolo wonder, while a DNA band admeasuring 494 bp was observed in the remaining 5 varieties. This polymorphism was used to discriminate non-pungent varieties namely California wonder and Yolo wonder from pungent varieties namely G-5, Pusa sadabahar, Pusa jyothi, LCA-335 and Kotpar. This method is useful to distinguish non-pungent varieties from pungent varieties, at seed level itself. BYLLA PRASAD, TOUTREDDY RADHA , ROHELA GULAB KHAN , CHITHAKARI RAVI AND THAMMIDALA CHRISTOPHER REUBEN Capsicum annuum L., Capsicum chinense, Jacq, MAP1 primer, zygotic embryo, genomic DNA, PCR. 889-892