10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) A time-course study was performed to assess lung concentrations and bioactivities of a fully human monoclonal antibody (mAb) targeting lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan (PA) flagella type b (LST-007) following intra-nasal administration. Intra-nasal administration of LST-007 (5 mg/kg) resulted in high mAb concentrations (11 mg/ml) within the bronchoalveolar lavage (BAL) fluid at 15 min post-administration, which decreased to 3 mg/ml at 4 hr. In marked contrast, LST-007 concentrations in blood were 110 and 11 fold lower at the same time points. Scrutinization of BAL fluid demonstrated the presence of both intact and immunoreactive LST-007 towards PAO1 bacteria, which impeded bacterial motility, underscoring the maintenance of biological activity. These compelling data lay credence that intra-nasal and thus potential inhalation modes of administration might represent lessThan i greaterThan bona fide lessThan /i greaterThan routes for a targeted mAb delivery to the lung environment and establishment of therapeutically effective concentrations. Such delivery approaches could help combat life-threatening, pneumonia infections caused by PA. AZMI ADAWI AND LEWIS F. NEVILLE Monoclonal antibody, intra-nasal, bronchoalveolar lavage; Pseudomonas aeruginosa 713-721