10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) A simple, fast, specific and precise LC-MS/MS method was developed and validated for the determination of zaltoprofen enantiomers in pharmaceutical formulation. The interface used was Atmospheric Pressure Chemical Ionisation Technique. Analysis was performed using a ACI cellu 1 column (150 x 4.6 mm I.D., particle size 5 μ) by isocratic elution with 0.1% Ammonia solution: Acetonitrile (10: 90 v/v) and flow rate was 0.5 ml/min. The calibration plot was linear over the range of 18-54 ng/ml of S-zaltoprofen and 22-66 ng/ml of R-zaltoprofen with a correlation coefficient of 0.999 for S and R zaltoprofen. This optimized mobile phase separated S-zaltoprofen at 2.4 min and R-zaltoprofen at 3.9 min respectively. The proposed LC-MS/MS method is suitable for analysis of zaltoprofen enantiomers in pharmaceutical formulations and quality control analysis. B. GOWRAMMA , S. N. MEYYANATHAN Zaltoprofen, LC-MS/MS, Atmospheric Pressure Chemical Ionisation, Enantiomers, Pharmaceutical formulation 883-889