10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) The development of new antimicrobial agents against resistant pathogens is of increasing interest. Therefore, the ethanolic extract from medicinal plant has been used traditionally as folk medicine over a period of time. The antimicrobial activity of lessThan i greaterThan Adhatoda vasica lessThan /i greaterThan was assessed against some pathogen viz. lessThan i greaterThan Proteus vulgaris lessThan /i greaterThan , lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan , lessThan i greaterThan Escherichia coli lessThan /i greaterThan and lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan by Agar disc diffusion method the antimicrobial activity was measured at different concentration of 100 µg, 200 µg, 300 µg, 400 µg, and 500 µg. The plant material was extracted by using Soxhlet extractor with ethanol solvent and ethanolic extract was screened phytochemically for the presence of secondary metabolites like alkoloids, phenolics, glycosides, tannins, flavonoids, saponins etc. SAGAR VIJAYRAO KATHALE Adhatoda vasica, Ethanolic extract, Secondary metabolites, Antimicrobial activity. 930-933