10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) lessThan i greaterThan Vernonia lessThan /i greaterThan lessThan i greaterThan anthelmintica lessThan /i greaterThan is a wild medicinal herb distributed throughout the India. It is used as a folk medicine against cough, skin diseases, diarrhea and fever. In the present study, ethanolic extract was obtained from seeds of lessThan i greaterThan Vernonia anthelmintica lessThan /i greaterThan and phytochemical analysis was done to screen alkaloids, saponins, steroids, carbohydrates, flavonoids, glycosides, polyphenols and gums and mucilage. Antimicrobial activity was carried out by using four bacterial strains lessThan i greaterThan Staphylococus aureus, E. coli, Pseudomonas aeruginosa, Bacillus subtilis lessThan /i greaterThan and two fungal strains such as lessThan i greaterThan Aspergillus niger lessThan /i greaterThan and lessThan i greaterThan Trichophyton rubrum. lessThan /i greaterThan This ethanolic extract showed good antibacterial activity against lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan (20.21±0.8mm) and lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan (19.25±0.6) whereas moderate activity against Staphylococcus aureus (15.34±0.5) and lessThan i greaterThan E. coli lessThan /i greaterThan (14.56±0.8). Whereas ethanolic extract showed good anti-fungal activity against Aspergillus flavus (19.64±1.8) and showed moderate activity against Trycophyton rubrum (13.21±1.1) at highest concentration of ethanolic extract 80mg/ml concentration. lessThan i greaterThan V.antihelmintica lessThan /i greaterThan seed ethanolic extract showed good free radical scavenging activity at all concentrations of ethanolic extract. Our results clearly saying that seed ethanolic extract showed high content of phytochemicals, highest antimicrobial and antioxidant activity and our results supported the usage of lessThan i greaterThan Vernonia anthelmintica lessThan /i greaterThan in folk and traditional medicine. CH.SANTOSH, IDRESS HAMAD ATTITALLAAND M.MADAN MOHAN Vernonia anthelmintica, phytochemicals, DPPH, Minimum Inhibitory concentration, antioxidant activity. 960-966