10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Present study explores the biosynthesis and characterization of gold nanoparticles using the ethanolic bract extract of banana lessThan i greaterThan (Musa balbisiana) lessThan /i greaterThan flower. On treatment of aqueous solutions of Chloroauric acid with lessThan i greaterThan M. balbisiana lessThan /i greaterThan leaf extract, stable gold nanoparticles were rapidly formed in room temperature within 1 hour. The gold nanoparticles were characterized by UV-visible spectroscopy, Transmission Electron Microscopy (TEM), X- ray Diffraction studies (XRD) and Fourier Transform Infra-red Spectroscopy (FTIR). Surface Plasmon Resonance Spectra for gold nanoparticles are obtained at 530nm with pink-red color. Further FTIR analysis was also done to identify the functional groups in gold nanoparticles. BORNALI BAISHYA AND MOHAN CHANDRA KALITA Musa balbisiana, bract, gold nanoparticles, X ray diffraction 873-881