10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) The present study established a protocol for assessing lessThan i greaterThan in vitro lessThan /i greaterThan chromium absorption potential of lessThan i greaterThan Alternanthera sessilis lessThan /i greaterThan . The study also investigated the effect of chromium on morphology of the micropropagated plants. Murashige and Skoog (1962) medium fortified with 0.5 mg/l of 6-benzylaminopurine (BAP) and 0.2 mg/l Indole-3 acetic acid (IAA) was selected as ' lessThan i greaterThan in vitro lessThan /i greaterThan plantlet induction medium (IVPI)' for lessThan i greaterThan A. sessilis lessThan /i greaterThan . Optimum chromium uptake (OCU) concentration was determined as 8 mg/l K lessThan sub greaterThan 2 lessThan /sub greaterThan Cr lessThan sub greaterThan 2 lessThan /sub greaterThan O lessThan sub greaterThan 7 lessThan /sub greaterThan on which moderate effects in length of shoot (4.5 cm) and root (1.2 cm) and survival rate (58.6%) was recorded. The chromium accumulation of lessThan i greaterThan A. sessilis lessThan /i greaterThan at OCU concentration is comparatively similar in both lessThan i greaterThan in vitro lessThan /i greaterThan condition (1011 μg/g in root; 201 μg/g in shoot) and Hydroponics (996 μg/g in root; 195 μg/g in shoot). The lessThan i greaterThan in vitro lessThan /i greaterThan method was found to be a valuable tool in identifying prospective phytoremediation candidates to reduce environmental toxic metals. SUBHALAKSHMI A, RAJAGOPAL K, NITHIYANANDHAN G, JAYANANDI DEVI AND KARTHIKEYAN S Phytoremediation, direct regeneration, chromium, environmental pollutants, detoxification 618-624