10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) In present investigations molecular characterization of seven Pieridae butterflies was carried out using four RAPD markers. A total 57 bands were scored with four decamer primers of which 57 were polymorphic and the percentage of polymorphism was 100%. Genus specific bands have been observed i.e. a band of 818bp with primer 12 & 520bp with primer OPP-5. Dendrogram constructed using the NTSYS lessThan sub greaterThan spc2.2 lessThan /sub greaterThan software divided the pierids into two clades. Cluster-I comprises of two species lessThan i greaterThan viz lessThan /i greaterThan . lessThan i greaterThan Pieris brassicae nepalensis lessThan /i greaterThan and lessThan i greaterThan Pieris canidia indica. lessThan /i greaterThan Cluster-II comprises of 5 species lessThan i greaterThan viz lessThan /i greaterThan . lessThan i greaterThan Colias electo fieldi lessThan /i greaterThan , lessThan i greaterThan Colias erate erate lessThan /i greaterThan , lessThan i greaterThan Gonepteryx rahmni nepalensis, lessThan /i greaterThan lessThan i greaterThan Pontia daplidice lessThan /i greaterThan lessThan i greaterThan moorei lessThan /i greaterThan & lessThan i greaterThan Terias hecabe fimbriata. lessThan /i greaterThan Cluster –II is subdivided into two, sub-cluster-I consisting of lessThan i greaterThan Colias electo fieldi lessThan /i greaterThan , lessThan i greaterThan Colias erate erate lessThan /i greaterThan , lessThan i greaterThan Terias hecabe fimbriata lessThan /i greaterThan & lessThan i greaterThan Gonepteryx rahmni nepalensis, lessThan /i greaterThan sub-cluster-II consists of only lessThan i greaterThan Pontia daplidice lessThan /i greaterThan lessThan i greaterThan moorei lessThan /i greaterThan . M.S.THAKUR Butterflies, Genetic diversity, Pieridae, RAPD-PCR 1090-1098