10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) Lignocellulosic material generated as agro-industrial wastes can be used as cheap and easily available substrates for the production of many industrial enzymes. A strain of lessThan i greaterThan Cladosporium tenuissimum, lessThan /i greaterThan capable of producing xyloglucanase using tamarind kernel powder as the sole carbon source, was isolated from a soil sample collected near Bangalore. Viscometric analysis showed that it is an endoglucanase with very low activity towards carboxymethylcellulose, suggesting that it might be a xyloglucan-specific endoglucanase. The enzyme was extracellular and produced preferentially at alkaline pH, thus enhancing its viability for commercial applications. Apart from xyloglucanase activity, the culture filtrate showed FPase, β-glucosidase and β-galactosidase activities. As per our knowledge, this is the first report on lessThan i greaterThan C. tenuissimum lessThan /i greaterThan as a producer of xyloglucanase. RASHMI R AND SIDDALINGAMURTHY K R Xyloglucanase; Cladosporium tenuissimum; Tamarind xyloglucan; β-glucosidase 1291-1297