10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 3 2013 (July - September) lessThan i greaterThan Saracca indica lessThan /i greaterThan is a terrestrial tree found in tropical rain fed areas across the world. In India it is mostly found in peninsular region and in the foot hills of Himalaya mountain range. Till date its bark has found tremendous pharmacological application due to the presence of sterols like ergosterol which is a precursor of certain prostaglandins. Further investigations so far have indicated good antimicrobial activity too but reports of the application of this are still scanty. The objective of this investigation was not only to see whether the leaf extract also had antimicrobial activity, but to characterize such properties. The work was carried out by noting the difference in growth patterns of two most common opportunistic pathogens like lessThan i greaterThan Escherichia coli lessThan /i greaterThan and lessThan i greaterThan Micrococcus aureus lessThan /i greaterThan . It was observed that the growth rate of these two organisms was reduced using the ethanolic extract. This was primarily due to the presence of myristic acid and palmitic acid in the ethanolic extract, as revealed in the GCMS study. There was no effect on the growth pattern of lessThan i greaterThan E. coli lessThan /i greaterThan by the aqueous extract. However, surprisingly, aqueous extract served as a growth stimulator for lessThan i greaterThan M. aureus. lessThan /i greaterThan This was because both these fatty acids were absent in the aqueous extract and there was good amount of readily assimilable reducing sugars present in the aqueous extract. PRIYANKA S. LOKHANDE, ANURADHA D. KARADGE, KUMAR S. PATIL, NAEIM H. NADAF AND JAI S. GHOSH Ashoka, antimicrobials, prostaglandins, E.coli, M.aureus. 1344-1347