10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) The present study deals with the assessment of antimicrobial activity (antibacterial, antifungal and antiyeast) of bio-oil from lessThan i greaterThan Pongamia glabra lessThan /i greaterThan , lessThan i greaterThan Mesua ferrea lessThan /i greaterThan and lessThan i greaterThan Parachlorella lessThan /i greaterThan spp deoiled cake. The bio-oil from the respective deoiled cakes were obtained via the process of pyrolysis (ambient to 500°C at a heating rate of 40°C/min) in a vertical tubular fixed bed reactor in nitrogen atmosphere. Bio-oil from lessThan i greaterThan Mesua ferrea lessThan /i greaterThan deoiled cake (BMFDC) and lessThan i greaterThan Pongamia glabra lessThan /i greaterThan deoiled cake (BPGDC) recorded the most effective Zone of Inhibition (ZOI) against lessThan i greaterThan S. aureus lessThan /i greaterThan viz., 28 and 29 mm respectively. BMFDC and BPGDC were more effective in terms of antimicrobial efficacy in contrast to bio-oil from lessThan i greaterThan Parachlorella lessThan /i greaterThan deoiled cake (BPCDC). The antimicrobial activity of the bio-oil samples may be due to the presence of phenolic and carboxyl groups (detectable by FTIR spectroscopy). The results of this study indicate the presence of bio-active agents in bio-oils which may lead to development of new pharmaceuticals. MAYUR. M. PHUKAN, RAHUL S. CHUTIA, RAHUL KUMAR, D. KALITA, B. K. KONWAR AND R. KATAKI Antimicrobial, De-oiled cake, Bio-oil, Microalgae 910-918