International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 4
2013 (October - December)
ISOLATION AND CHARACTERIZATION OF AN AZO DYE REACTIVE RED 2 DEGRADING BACTERIA FROM DYE CONTAMINATED SOIL
A bacterial strain GSM3, capable of decolorizing an azo dye Reactive Red 2 was isolated from dye contaminated soil sample collected from Ammu dyeing industry, Bellary, India. Based on phenotypic characteristics and 16S rDNA sequencing, strain GSM3 was identified as lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan GSM3. Isolate GSM3 showed 97% decolorization of Reactive Red 2 (300 mgL lessThan sup greaterThan ─1 lessThan /sup greaterThan ) within 10 h, while maximally it could decolorize 2 gL lessThan sup greaterThan ─1 lessThan /sup greaterThan of dye within 62 h with 75% decolorization in carbon free mineral salts medium supplemented with 0.1% (w/v) of yeast extract as a cosubstrate under static condition. The isolate has shown good decolorization activity over a wide range of pH from 5.0 to 9.0 and temperature 30 lessThan sup greaterThan o lessThan /sup greaterThan C to 45 lessThan sup greaterThan o lessThan /sup greaterThan C respectively. However, highest decolorization was observed at 37 lessThan sup greaterThan o lessThan /sup greaterThan C and pH 7.0. UV lessThan b greaterThan - lessThan /b greaterThan vis analyses and colorless bacterial cells suggested that the isolate GSM3 exhibited decolorizing activity through biodegradation, rather than inactive surface adsorption.Thus, the isolate GSM3 had great potential to be applied in biodegradation of dye effluents treatment.
MALLIKARJUN C, BHEEMARADDI, CHANNAPPA T. SHIVANNAVARAND SUBHASCHANDRA M. GADDAD
Reactive Red 2, Azo dye, Decolorization, Degradation and Pseudomonas aeruginosa GSM3
711-722