10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 4 2013 (October - December) Lipase production in lessThan i greaterThan Aspergillus niger lessThan /i greaterThan was tested using both submerged fermentation (SmF) and solid-state fermentation (SSF) on a mineral culture medium and wheat bran, respectively.Culture media was optimized in both SmF and SSF. We found 1.46 IU/mL was the optimum activity of lipase in case of submerged fermentation, when medium containing 2% glucose and 2% olive oil under the conditions of 1 vvm and 450 m lessThan sup greaterThan -1. lessThan /sup greaterThan However, 9.14 IU/g of dry solid substrate equivalent to 4.8 IU/mL of lipase activity was reached using solid-state fermentation process with a medium containing 0.75 % of ammonium sulphate and 0.34 % of urea. The optimum pH and temperature for enzymatic activity were pH=6 and 40 °C, respectively. When we used neutral and midly acid media, temperature ranges between 20 to 30⁰C for 24 hour, the enzyme showed 80% of its initial activity. DIPTENDU SARKAR AND SOUMITA LAHA Aspergillus niger, solid-state fermentation, lipases 978-985