10.22376/ijpbs.2019.10.1.p1-12 Volume 1 Issue 4 2010 (October - December) An efficient and reproducible protocol for Agrobacterium- mediated transformation of an agronomically useful lessThan i greaterThan Oryza sativa lessThan /i greaterThan variety, Pusa Basmati- 1 (IET- 10364) has been standardized. Although several reporters have been claimed the Agrobacterium- mediated transformation but here we studied the effect of acetosyringone and age of callus on transformation frequency for scutellum- derived callus of Pusa basmati- 1. By optimizing these two parameters for Agrobaterium- mediated transformation, we can produce an efficient and reproducible protocol. The callus derived from mature rice seeds were used as explants for transformation studies. Callus were co-cultured with lessThan i greaterThan Agrobacterium tumefaciens EHA 105 lessThan /i greaterThan harboring a binary vector p35SGUSINT that carried Beta- glucuronidase ( lessThan i greaterThan uid A lessThan /i greaterThan ) and It contains gene lessThan i greaterThan npt II lessThan /i greaterThan , determining resistance to kanamycin, under the control of the constitutive ( lessThan i greaterThan nos lessThan /i greaterThan ) promoter. Maximum Agrobacterium- mediated transformation frequency was observed at 350mM/l acetosyringone with 35 days old callus. R. M. Tripathi,H. S. Bisht,R.P. Singh Pusa Basmati – 1, Beta- glucuronidase, Transformation, Agrobacterium tumefaciens EHA105, p35SGUSINT and Callus transformation. 163-171