International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 2
2014 (April - June)
ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF TERPENOID FRACTION ISOLATED FROM THE SHOOT OF Plectranthus hadiensis
In the present study, antioxidant and anti-inflammatory activities of terpenoid fraction isolated from shoot of P. hadiensis was investigated. The terpenoid fraction was obtained from the aqueous ethanolic extract by liquid-liquid partitioning using Ethyl acetate as solvent. The terpenoid fraction thus obtained was concentrated and taken for further analysis and bioassays. The terpenoid fraction (IC50 22.76 ± 0.787 µg/mL) exhibited excellent free radical scavenging capacity towards 1, 1 –diphenyl – 2- picrylhydrazyl stable free radical (DPPH·). The reducing power of the fraction and BHT, which was taken as the standard increased with increasing concentration. The nitric oxide radical scavenging capacity of the fraction (IC50 79.84 ± 0.519 µg/mL) of P. hadiensis is comparable to that of the standard Ascorbic acid (IC50 75.81 ± 0.754 µg/mL). The results showed that the terpenoid fraction isolated from the shoot of P. hadiensis had a strong reductive capacity. The in vitro anti inflammatory assays included BSA denaturation inhibition, HRBC membrane stabilization and Platelet aggregation inhibition assays. The fraction showed IC50 values of 56.18±0.766 µg/mL, 57.17±0.890 µg/mL and 54.26±0.744 µg/mL for BSA denaturation inhibition, HRBC membrane stabilization and Platelet aggregation inhibition assays respectively, which was compared with the standard non-steroidal drug, viz., Diclofenac sodium. The study demonstrated that the terpenoid fraction efficiently scavenged DPPH, OHË™ and NO radicals. The anti-inflammatory activity of the fraction showed that it can be positively compared to the standard drug used for treating inflammation-related ailments. The results of the present study revealed that the terpenoid fraction obtained from the shoot of P. hadiensis has potent antioxidant and anti-inflammatory activity. lessThan br / greaterThan
DARSAN. B. MENON, SASIKUMAR J.M AND GOPALAKRISHNAN V.K
P. hadiensis, Terpenoid fraction, Antioxidant activity, Anti-inflammatory activity.
197-205