International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 2
2014 (April - June)
DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY
Embryos of Lycopersicon esculentum Mill cv Pusa Ruby cultured on MS medium supplemented with auxins such as IAA (1.0mg/l) and NAA (1.0mg/l) alone produced whole plantlets. However, embryos cultured on 2,4-D (1.0mg/l) produced callus with roots. BAP (1.0mg/l) and Kn (1.0mg/l) alone are able to induce shoots with callus. Among various combinations of auxins and cytokinins i.e., (1.0 mg/l) IAA + BAP (2.0 mg/l) and IAA (1.0 mg/l) + Kn (2.0 mg/l) gave good response and produced green nodular callus with shoots. Whereas, NAA (1.0mg/l) + BAP (2.0mg/l) and Kn (2.0mg/l) produced callus only. The combinations of IAA (0.5mg/L) + BAP (4.0 mg/L) was found to be better medium compared to all other concentrations of IAA + Kn medium analyzed. lessThan br / greaterThan
P. KARUNAKAR RAO
Lycopersicon esculentum Mill cv Pusa Ruby Direct regeneration, Embryoculture, Morphogenic response growth regulators.
929-933