10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 2 2014 (April - June) A new series of 2,5-disubstituted 1,3,4-thiadiazoles were synthesized by using different aromatic or aliphatic carboxylic acids (1) and thiosemicarbazide (2). The structures of the synthesized compounds were established by their spectral (IR, 1H NMR and Mass) data. All the compounds were screened for their antimicrobial activity against Staphylococcus aureus. Escherichia coli, Compounds showed mild to moderate activity, but not comparable with the standard (Norflaxacin).  AMGOTH SRINIVAS NAYAK 1,3,4-thiadiazole, Synthesis, Antimicrobial activity, Cup plate method. 344-347