10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) A rapid, sensitive and specific high-performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative determination of free Paroxetine (PAX) in human serum. Densitometric analysis of free PAX was carried at 293nm after simple liquid-liquid extraction. The method uses HPTLC aluminium plates pre-coated with silica gel G 60F lessThan sub greaterThan 254 lessThan /sub greaterThan as stationary phase and acetone: carbon tetrachloride: triethylamine (5:5:0.3,v/v/v) as mobile phase for the separation of free PAX from serum constituents. The calibration curve was linear (r lessThan sup greaterThan 2 lessThan /sup greaterThan = 0.996) in the tested range of 95-240 ng spot lessThan sup greaterThan –1 lessThan /sup greaterThan with a limit of detection (LOD) 30 ng spot lessThan sup greaterThan –1 lessThan /sup greaterThan and limit of quantification of (LOQ) 95 ng spot lessThan sup greaterThan –1 lessThan /sup greaterThan . Free drug recovery from serum averaged 94.4% to 99.28%. Intra- and inter-day precision (% RSD) values were PRATHIKANTAM PUSHPALATHA , RAJENDRA KUMAR SARIN , MOHD IDRIS , MESINENI ANAND RAO , TULSIDAS RAMACHANDRA RAO BAGGI AND M.SATISH VARMA Paroxetine, High Performance Thin Layer Chromatography, Quantitative determination, Human serum, Interference, Densitometry 01-08