10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) The indiscriminate use of commercial antimicrobial drugs has resulted in multiple drug resistance and adverse effects on the host. The present study was aimed to elucidate the effect of different biotic/abiotic elicitors on the growth as well as to enhance the metabolite production in leaf and epicotyl calli of lessThan i greaterThan Nigella sativa. lessThan /i greaterThan Further, antibacterial activity of these callus extracts was evaluated against five Gram positive and Gram negative bacteria. TLC data confirmed the presence of alkaloids, phenols, flavonoids, glycosides and importantly terpenoids in control and elicitated callus extracts. Calculated MIC values showed that maximum inhibition was caused by MnCl lessThan sub greaterThan 2 lessThan /sub greaterThan (1.08±0.3 µg ml lessThan sup greaterThan -1 lessThan /sup greaterThan ) lessThan sub greaterThan , lessThan /sub greaterThan pectin (1.28±0.5 µg ml lessThan sup greaterThan -1 lessThan /sup greaterThan ) and control epicotyl callus (2.02±0.3 µg ml lessThan sup greaterThan -1 lessThan /sup greaterThan ) and minimum by CoCl lessThan sub greaterThan 2 lessThan /sub greaterThan and cellulose leaf callus where lessThan i greaterThan E. coli lessThan /i greaterThan being most sensitive followed by lessThan i greaterThan S. typhi, B. cereus, S. aureus lessThan /i greaterThan lessThan i greaterThan K. pneumoniae lessThan /i greaterThan towards the extracts. Results showed that the extracts were better antibacterial agents when compared with commercially available antibiotics. HERA CHAUDHRY, NIDA FATIMA AND IFFAT ZAREEN AHMAD Callus, elicitors, phytochemicals, TLC, antibiotics 903-916