10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) Aqueous leaf extract of lessThan i greaterThan Luffa acutangula lessThan /i greaterThan Roxb. Var. lessThan i greaterThan amara lessThan /i greaterThan . Lin. was used for the synthesis of silver nanoparticles using antibacterial activity. The silver nanoparticles formation was confirmed by the color change of plant extracts (SNPs) and further confirmed with the help of UV – Vis spectroscopy and Fourier Transform Infra Red (FTIR). The absorption spectra of silver nanoparticles studied using the UV-VIS spectroscopy had absorbance peak at 420 nm. The antibacterial activities of silver nanoparticles were studied using human pathogenic bacterial strains was used in lessThan i greaterThan Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella paratyphi, lessThan /i greaterThan and lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan . The zone of inhibition was higher in lessThan i greaterThan Klebsiella pneumonia lessThan /i greaterThan and zone of inhibition lower in lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan . The move towards an extracellular synthesis of Ag lessThan sup greaterThan + lessThan /sup greaterThan nanoparticles using dried biomass appears to be cost effective, eco-friendly method of nanoparticles synthesis. R. SYED MOIDEEN AND A. LAKSHMI PRABHA Luffa acutangula, Silver nitrate, UV, FTIR, FE-SEM, Microorganisms. 1051-1061