10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) The genus  lessThan i greaterThan Streptomyces lessThan /i greaterThan  is represented in nature by the largest number of species and varieties among the family Actinomycetaceae. This study aims to isolate and characterize novel strains of Streptomyces with high antimicrobial and antifungal production capability. The morphological , biochemical and biological characteristics of marine actinomycetes isolated from the coast of the red sea of Seini , Egypt, are presented. A total of 50actinomycetes isolates were isolated by serial dilution plate technique, of these 20 isolates showed activity in primary screening against test pathogens used in this study.Antimicrobial activity of cul­tivation medium was determined using the diffusion - disc method. As test microorganisms lessThan i greaterThan Bacillus Subtilis lessThan /i greaterThan , lessThan i greaterThan Bacillus cereus lessThan /i greaterThan , lessThan i greaterThan Escherichia coli,Pseudomonasaeruginosa lessThan /i greaterThan , lessThan i greaterThan Lactobacillus casei lessThan /i greaterThan and lessThan i greaterThan Candida albicans, lessThan /i greaterThan are applied. The same isolates showed inhibitory effects against pathogenic fungi i.e. lessThan i greaterThan Alternariaalternata lessThan /i greaterThan lessThan i greaterThan , A. solani lessThan /i greaterThan , lessThan i greaterThan Colletotrichumgloeosporioides, Fusariumoxysporum, Aspergillusflavus lessThan /i greaterThan and lessThan i greaterThan Aspergillusniger lessThan /i greaterThan . This may indicate the same compound was responsible for the antibacterial activity of the actinomycetes. Out of 20 isolates, 13 isolates exhibited both antibacterial and antifungal activity.Finally potent actinomycetalisolate were selected and it's tested for enzymes production. The isolates were produced most important hydrolytic enzymes as chitinase and β-1, 3 glucanase , cellulose and protease. The culture filtrate of these strains were effective against Alternariaalternata lessThan i greaterThan , A. solani lessThan /i greaterThan and lessThan i greaterThan Colletotrichumgloeosporioides lessThan /i greaterThan in tomato under greenhouse conditions. WAFAA M. HAGGAG AND RADWANEL-SEHEMY I.A. Antifungal, antimicrobial activities, secondary metabolites,Streptomyces sp., 527-536