10.22376/ijpbs.2019.10.1.p1-12 Volume 5 Issue 4 2014 (October - December) In the present study, lessThan i greaterThan Hypocrea lixii lessThan /i greaterThan SURT01, a isolate from poultry soil was subjected to 18s rRNA analysis was submitted to NCBI – Genbank. The strain was induced in minimal media which was found to produce the phytase enzyme. The characterization of extracellular phytase showed optimum activity at pH of 6.5 and temperature of 50ºC after an incubation for 120 hours. Different nitrogen sources were screened and peptone showed more enhancements in the enzyme production. Time course study was done on the culture media and that showed 120 hours to reach maximum activity. The enzyme was partially purified by 60% by ammonium sulphate precipitation and gel permeation chromatography was done to get different fractions. SDS PAGE was carried out to understand the molecular weight of the protein. R. THYAGARAJAN , S. KARTHICK RAJA NAMASIVAYAM AND G. NARENDRAKUMAR Phytase, Purification, Ammonium sulphate, Gel permeation chromatography, SDS PAGE. 680-687