10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) Multiplication of antibiotic resistant bacteria complicates the wound healing process nowadays. It has been proved that our ancient medicine honey is a panacea for a lot of diseases and it plays a vital role in wound healing. Hydrogel sheets were developed from chitosan and chitosan-honey in this study to evaluate the required properties for effective wound dressings, such as, folding endurance, degradation, water vapour transmission, swelling ratio, tensile strength, elongation, antimicrobial property and animal study. Based on the Minimum Inhibition Concentration (MIC) value of Manuka honey ( lessThan i greaterThan Leptospermum scoparium lessThan /i greaterThan ), 8% concentration of honey was experimented. The hydrogel sheets were produced from chitosan(C) and chitosan-honey (CH) with lactic acid as solvent. The CH sample showed significant results in physical properties and mechanical properties. It also showed higher zone of inhibition against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Escherichia coli, lessThan /i greaterThan the most wound infecting bacteria and contributed significantly to excision wound healing with 94.4% wound contraction on 12 lessThan sup greaterThan th lessThan /sup greaterThan day. The sheet was also soft, flexible and exhibits its potential to be used as a wound dressing. L.SASIKALA AND BHAARATHI DURAI Animal Study, Chitosan Film, Hydrogel Sheet, Leptospermum Scoparium, Manuka Honey, Wound dressing. 26-37