10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) lessThan i greaterThan Umbilicus rupestris lessThan /i greaterThan (UR) is a medicinal plant used in traditional medicine against inflammation and irritation of the skin. The aim of this work is to study lessThan i greaterThan in vivo lessThan /i greaterThan on Wistar rats the acute and subacute toxicity of the methanolic extract of lessThan i greaterThan U. rupestris lessThan /i greaterThan (URMeOH ), anti -inflammatory effects by using several methods : The inflammation induced by carrageenan, the inflammation induced by the inflammation mediators, arthritis , peritonitis in Wistar rats and finally, effect of URMeOH on the stabilization of erythrocyte membrane lessThan i greaterThan in vitro lessThan /i greaterThan . In the acute toxicity study, the LD50 of URMeOH was found higher than 2000 mg / kg b.w ; and this dose did not cause neither death nor signs of toxic manifestations on treated rats . In the subacute toxicity study , no toxic signs were observed after 28 days of treatment with therapeutic doses 100 and 200 mg / kg b.w. The inflammation induced by carrageenan, chemical mediators (histamine and serotonin) and formalin solution was significantly inhibited and dose-dependent manner compared to the negative control after the administration of URMeOH (100 mg and 200 mg / kg B.w.). Also, the URMeOH revealed that it has an effect on the stabilisation of erythrocyte membrane after induction of hemolysis and it has the anti-peritonitis activity by inhibiting leukocytes migration.This study demonstrated the anti -inflammatory effects of URMeOH and that confirmed its use in traditional medicine. AFAFBENHOUDA AND MOULOUD YAHIA Umbilicus rupestris, methanolic extract, toxicity, paw edema, leukocytes migration; anti-inflammatory activity. 395-408