10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) lessThan i greaterThan Justicia glauca lessThan /i greaterThan Rottler lessThan i greaterThan , lessThan /i greaterThan a medicinal plant belongs to dicotyledonous flowering plants family Acanthaceae. Plants from this family have been reported in the scientific literature to possess promising medicinal properties. The preliminary phytochemical analysis and antibacterial activity of lessThan i greaterThan Justicia glauca lessThan /i greaterThan Rottler total plant extracts were carried out using different organic solvents against some pathogenic bacteria viz., lessThan i greaterThan Escherichia coli, Klebsiella pneumonia, Bacillus subtilis lessThan /i greaterThan and lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan by agar well diffusion method. Results indicated that the petroleum ether, chloroform and ethanol extracts of lessThan i greaterThan Justicia glauca lessThan /i greaterThan Rottler showed significant growth inhibitory activity against lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan , lessThan i greaterThan E. coli lessThan /i greaterThan and lessThan i greaterThan Klebsiella pneumonia lessThan /i greaterThan at 100mg/ml concentration. The most active extracts were compared with the standard antibiotic Ampicillin. The highest zone of inhibition was recorded (13mm) at 100mg/ml concentration of petroleum ether and ethanol lessThan i greaterThan Klebsiella pneumonia lessThan /i greaterThan and lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan respectively. This study revealed that the lessThan i greaterThan Justiscia glauca lessThan /i greaterThan Rottler have a potential broad spectrum antibacterial activity. Combinations of extracts may be the best herbal antibiotics.  ARUN JYOTHI BHEEMAGANI, CHAKRAPANI PULLAGUMMI,HANDRA SEKHAR SINGH BHALUDRA, NIRMALA BABU RAO AND ANUPALLI ROJA RANI Justiscia glauca Rottler, Phytochemicals, well diffusion, zone of inhibition, herbal antibiotics. 409-415