10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of lessThan i greaterThan Canavalia gladiata lessThan /i greaterThan (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L – 1.5mg/L) and Indole acetic acid (IAA) (0.5mg/L – 1.5mg/L) for shoot induction. The best shoot proliferation was observed at the concentration of 1.0mg/L of BAP and 1.0 mg/L of IAA from only nodal explants of lessThan i greaterThan Canavalia gladiata lessThan /i greaterThan after 4 weeks of culture. Combination of PGRs (BAP and IAA) was found to be more effective for shoot induction of nodal explants of lessThan i greaterThan Canavalia gladiata lessThan /i greaterThan . For callus regeneration the nodal explants of lessThan i greaterThan Canavalia gladiata lessThan /i greaterThan were used and the MS medium was supplemented with various concentration of α-naphthalene acetic acid (NAA) (0.5mg/L – 3.0mg/L). Highest callus induction was observed at the concentration of 1.5mg/L of NAA, and in this concentration highly desirable creamy white friable callus was observed. D.SURESH, T.SILAMBARASAN AND R. DHANDAPANI Micro propagation, In vitro regeneration, Callus induction, Forisome, Plant growth regulators, 6-benzyl amino purine, indole acetic acid, α-naphthalene acetic acid. 440-445