10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) This research work reports the homology modeling of acid phosphatase, phytase and alkaline phosphatase genes of lessThan i greaterThan Aspergillus fumigatus lessThan /i greaterThan . Since soil is a reservoir for the isolation of such fungi, soil microbes were screened for phosphatases and a strain of lessThan i greaterThan Aspergillus fumigatus lessThan /i greaterThan was isolated. A typical fungal colony was isolated after serial dilution, identified as lessThan i greaterThan A. fumigatus lessThan /i greaterThan was used in this study. On molecular identification, the strains identified on the basis of morphological and physiological features were confirmed to belong to lessThan i greaterThan A. fumigatus lessThan /i greaterThan . Their ITS1-ITS4 sequences were up to 99% homologous to reference strains of lessThan i greaterThan A. fumigatus lessThan /i greaterThan from the GenBank. Acid phosphatase, alkaline phosphatase and phytase cDNAs were synthesized from lessThan i greaterThan Aspergillus fumigatus lessThan /i greaterThan RNA by PCR reactions using primers (designed from published NCBI sequences) with reverse transcriptase. This resulted in amplicons corresponding to the predicted gene size of the respective enzymes. They were cloned into bacteria and were subjected to automated DNA sequencing. DNA sequencing after plasmid isolation revealed that the entire nucleotide sequence of the alkaline phosphatase, acid phosphatase and phytase genes from genomic DNA of lessThan i greaterThan A. fumigatus lessThan /i greaterThan was 99.9 % similar to NCBI sequences of lessThan i greaterThan Aspergillus fumigatus. lessThan /i greaterThan The closest homolog to alkaline phosphatase gene of lessThan i greaterThan A. fumigatus lessThan /i greaterThan was lessThan i greaterThan Neosartorya fumigata lessThan /i greaterThan and lessThan i greaterThan Neosartorya fumigata. lessThan /i greaterThan The closest homolog to acid phosphatase gene of lessThan i greaterThan A. fumigatus lessThan /i greaterThan was lessThan i greaterThan Neosartorya fischeri. lessThan /i greaterThan The closest homolog to phytase gene lessThan i greaterThan of A. fumigatus lessThan /i greaterThan was lessThan i greaterThan Neosartorya fumigata lessThan /i greaterThan . NEELAKANTAN SURESH AND ARUNAVA DAS Alkaline phosphatase, Acid phosphatase, Phytase, Homology modelling, In silico 553-566