10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) Enterococci are increasingly recognized as an important nosocomial pathogen. The present study was done to establish the changing paradigm of blood culture enterococcal isolates and emergence of lessThan i greaterThan Enterococcus faecium lessThan /i greaterThan as a nosocomial pathogen. A total of 150 Enterococcal isolates were obtained during the study period from January 2013 to March 2014. 68.3% and 31.7% of adult and 53% and 43% of paediatric blood culture enterococcal isolates were lessThan i greaterThan Enterococcus faecalis lessThan /i greaterThan and lessThan i greaterThan Enterococcus faecium lessThan /i greaterThan respectively. Antimicrobial susceptibility testing by Kirby Bauer disc diffusion revealed vancomycin resistance of 7.2% and 1.8% for adult 15.3% and 26 % among the paediatric lessThan i greaterThan E. faecalis lessThan /i greaterThan and lessThan i greaterThan E. faecium lessThan /i greaterThan isolates respectively. The present study demonstrates the changing spectrum of enterococcal species causing bacteremia and increasing prevalence of vancomycin resistance among lessThan i greaterThan E. faecium lessThan /i greaterThan isolates. This emphasises the need for speciation of various enterococcal isolates and knowledge of antimicrobial resistance profile is necessary for management of serious enterococcal infections. MAANASA M BHASKAR AND HARISH B. N VRE – Vancomycin resistant enterococci, HLG- high level gentamicin, MDR- multidrug resistant, CLSI- clinical and laboratory standard institute, MIC- minimum inhibitory concentration, 579-584