International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 6 Issue 1
2015 (January - March)
VIRULENCE AND MOLECULAR CHARACTERIZATION OF AEROMONAS SPP. FROM FISH SAMPLES USING ARDRA TECHNIQUE
The detection of Virulence factors of lessThan i greaterThan Aeromonas lessThan /i greaterThan spp. is a key component in determining potential pathogenicity because these factors act Multifuntionally and Multifactorially. In this study, totally 50 carp fish samples were collected, Among these, 8 samples were found to be positive for lessThan i greaterThan Aeromonas hydrophila lessThan /i greaterThan and 13 were found to be positive for lessThan i greaterThan Aeromonas salmonicida. lessThan /i greaterThan Antibiotic resistant patterns were performed by Bauer-Kirby method. Both the species of lessThan i greaterThan Aeromonas lessThan /i greaterThan showed the highest resistance patterns to Amoxicillin, Ampicillin/Cloxacillin, Cefotaxime, and Clarithromycin. This study showed a virulence character of lessThan i greaterThan Aeromonas hydrophila lessThan /i greaterThan (8 isolates) positive for proteolytic (62.5%), lipolytic (75%), and β-Lactamase (100%), hemolytic (31.5%), slime production (100%). In lessThan i greaterThan Aeromonas salmonicida lessThan /i greaterThan (13 isolates) showed positive for proteolytic (61.5%), lipolytic (69.2%), and β-Lactamase (84.6%), and slime production (100%) respectively. lessThan i greaterThan Aeromonas lessThan /i greaterThan spp. isolated from fish samples were genetically characterized by Amplified Ribosomal DNA Restriction Analysis (ARDRA) of 16S rDNA gene by using lessThan i greaterThan EcoRI lessThan /i greaterThan enzyme, previously proposed as a useful tool to identify lessThan i greaterThan Aeromonas lessThan /i greaterThan spp. This present study revealed that the virulence and molecular characterization of lessThan i greaterThan Aeromonas lessThan /i greaterThan spp., is very important to detect the infection in fresh water fish, it should be essential to study the environmental factors, immunosuppressive factors and other adaptability factors of host and pathogen responsible for the establishment of pathogenic state.
S.THENMOZHI, P.VIJAYALAKSHMI, K.MOORTHY AND B.T.SURESHKUMAR
Aeromonas spp.,Virulence factors, Antibiotic resistance, 16SrDNA, ARDRA
593-603