10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) Bio prospecting of plants with anti-proliferative activity has been a major focus on the search of plants based cure. The pharmacological importance of lessThan i greaterThan Ceropegia lessThan /i greaterThan species (Apocynaceae) is mainly due to the presence of a pyridine alkaloid ' lessThan i greaterThan Ceropegin' lessThan /i greaterThan a potential antipyretic, analgesic, local anesthetic, antiulcer, mast-cell stabilizing, hepato-protective, tranquilizing, and hypotensive. Use of plant derived compounds as potent anticancer agents is of recent research interest. In the continuing effort to screen lessThan i greaterThan Ceropegia lessThan /i greaterThan species ( lessThan i greaterThan Ceropegia spiralis, Ceropegia juncea, lessThan /i greaterThan and lessThan i greaterThan Ceropegia candelabrum ) lessThan /i greaterThan for anticancer activity, plants were collected, micro propagated and hardening. lessThan i greaterThan In-vitro lessThan /i greaterThan propagated plants are collected, extracts tested for the activity using cell lines namely HCT-118 (Colon cancer cell) of the extracts from three species of same families tested (10,50,and100µg/ml). 10ug of lessThan i greaterThan Ceropegia spiralis lessThan /i greaterThan showed 47.15% of cell death, which can be considered significant lessThan i greaterThan . lessThan /i greaterThan Apoptosis was confirmed by acridine orange (AO) ethidium bromide (EB) fluorescent double staining which confirms the potent anticancer effect of ethyl acetate fraction of lessThan i greaterThan Ceropegia spiralis lessThan /i greaterThan . BINISH T AND MARY SUJA R Ceropegia spiralis, Ceropegia juncea, Ceropegia candelabrum, Anti-proliferative effect, HCT-118 Colon lines. 899-904