10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) This research focuses the phytochemical study of aqueous extracts (cold and hot) and ethanolic extract (70%) of lessThan i greaterThan Ocimum Sanctum L lessThan /i greaterThan . that are collected from local markets in Iraq. The investigation of phytochemical constituents involved the qualitative and quantitative studies. The results for qualitative analysis explained that the aqueous hot extract and ethanolic extract contains more amounts of active components such as flavonoids, saponins, tannins, alkaloids, terpenoids, glycosides and amino acids or primary and secondary amines rather than cold extract, also the quantitative analysis illustrated that the aqueous hot and ethanolic extracts contains more amounts of flavonoids, saponins, tannins and alkaloids because the aqueous hot and ethanolic extracts still have high yield from all active components and this results may be due to the role of heating in the hot extract and the type of solvent in the ethanolic extract of these active components that cannot be extracted by cold water. Biological activity was also analyzed for the aqueous and ethanolic extracts of lessThan i greaterThan Ocimum Sanctum L lessThan /i greaterThan . by using four different bacterial strains ( lessThan i greaterThan Staphylococcus aures lessThan /i greaterThan , lessThan i greaterThan Streptococcus pyogenes, Salmonella typhi lessThan /i greaterThan and lessThan i greaterThan Klebsiella) lessThan /i greaterThan and using agar diffusion method. The results showed that ethanolic extract has the biggest zone of inhibition for lessThan i greaterThan Staphylococcus aures lessThan /i greaterThan , lessThan i greaterThan Streptococcus pyogenes lessThan /i greaterThan and lessThan i greaterThan Klebsiella lessThan /i greaterThan while the aqueous cold and hot extracts given less results of inhibition zone. SARAH S.M. AL-TEMIMI AND LAMIA A.M.AL-MASHHEDY Ocimum sanctum L., phytochemical constituents, flavonoids, Saponins, anthroquinones, alkaloids, phlobatanins, Zone of inhibition. 999-1007