10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 1 2015 (January - March) Present research work was initiated role of BAP (benzyl amino purine),IAA and 2, 4-Don growth of callus and formation of multiple shoots by using shoot tip, leaf, and nodal explant. lessThan i greaterThan In vitro lessThan /i greaterThan shoot tips, leaf and nodal segment explants were inoculated on MS media supplemented with different concentrations of BAP and IAA (Indole-3- acetic acid) to produce multiple shoots as well as Calli. Shoot tip explants inoculated on MS medium supplemented of2.6 mg/LBAP combination with 0.4 mg/LIAA produce the highest number of shoots per explant was observed. The average numbers of shoot per explants were observed 2.5 mg/L and 0.2 mg/L IBA and IAA respectively. However, nodal segments of lessThan i greaterThan C. reticulate lessThan /i greaterThan inoculated on MS media containing 2 mg/LBAP and 1.5 mg/L IAA responsible for induction of callus was also observed. VISHWANATH WAGHMARE AND NARAYAN PANDHURE In vitro, Citrus reticulata, callus induction, Shoot Regeneration. 1275-1280