10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 2 2015 (April - June) lessThan i greaterThan Solanum nigrum lessThan /i greaterThan (Black Nightshade) is a medicinal plant member of the Solanaceae family of plants. This family comprises many genera, well known for their therapeutic properties. In addition to lessThan i greaterThan S. nigrum lessThan /i greaterThan , this family includes fruits and vegetables such as potato ( lessThan i greaterThan Solanum tuberosum lessThan /i greaterThan ), tomato, peppers, ornamental plants such as petunia, and other medicinal plants lessThan sup greaterThan 1 lessThan /sup greaterThan .The present work is focused on the phytochemical analysis and of aqueous, methanolic and hexane extracts of lessThan i greaterThan Solanum nigrum. lessThan /i greaterThan And screening for there antimicrobial activity against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Candida albicans lessThan /i greaterThan by well diffusion method measuring the zone of inhibition by turbidity method. This study assures that methanol is the best available solvent for phytochemical extraction and also it confirms the antimicrobial activity of the plant extract. Y.RAJRANA Antimicrobial screening, Candida albicans, Phytochemical analysis, Solanumnigrum, Staphylococcus aureus. 545-550