International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 6 Issue 2
2015 (April - June)
EFFECT OF IRAQI ETHANOLIC PROPOLIS EXTRACTS ON THE REACTIVE NITROGEN SPECIES AND GENOTYPE ALTERATION IN DIABETIC NEPHROPATHY RATS
The purpose of this research is to identify the effect of the Iraqi extract ethanolic propolis(EEP) on the nitrogen radicals and most important of which Peroxynitrite and nitric oxide, in rat laboratory infected kidney failure as a result of diabetes induce by streptozotocin (60 mg / kg), as well as its effect on the candidate genes in diabetic nephropathy are glucose transporter1(GLUT1) and nephrin promoter were found local EEP cannot reduced genotoxicity for STZ, but an effective influence on the free radicals where found a decrease in the concentration of Peroxynitrite radical was detected by detection of Nitrotyrosine (3- NT) by (ELISA), where the concentration increasing in diabetic groups while groups that treated by local EEP showed a decrease in the concentration of (3-NT) compared with control group, which indicates the low concentration of Peroxynitrite. As well as the results show the effect of local EEP on the low concentration of nitric oxide, and reduce the level of glucose in the blood, kidney function (creatinine, uric acid) and the presence of an improvement in the pathological changes of kidney tissues in the treated groups compared to the diabetic, this indicates that propolis is a strong immunological factors and antioxidant. The results we have obtained through this study to conclude that propolis cannot reduce the genotoxicity induced STZ but has immunological properties through its effect on reducing the concentration of 3-NT and NO lessThan b greaterThan lessThan sup greaterThan . lessThan /sup greaterThan lessThan /b greaterThan lessThan sup greaterThan • lessThan /sup greaterThan in Pre-treated and Post-treated groups and this is due to contain rich in flavonoids, phenolic acid, and others. It also has anti-oxidant properties through its effect anti-hyperglycemia and his effect moderate on kidney function by reducing the concentration of creatinine and uric acid in the blood through the improvement of histological changes in the kidney of rats with diabetes induced by STZ.
AMNA N. ZGHAIR AND FERDOUS A. JABIR
GLUT1, Nephrin promoter, EEP, 3-NT, Peroxynitrite and Mutation.
1339-1351