10.22376/ijpbs.2019.10.1.p1-12 Volume 1 Issue 2 2010 (April - June) Efficient method for isolation of high quality genomic DNA is the first step in the development of DNA based markers for fingerprinting and genetic diversity of crops. We have described a simple and user friendly protocol for extraction of DNA from dried sugarcane leaf samples, which dose not require any use of liquid nitrogen, making it advantageous over other common protocols. The DNA obtained using this procedure were consistently amplifiable, using both RAPD primers as well as ISSR primers, making it most useful in genetic diversity analysis of the sugarcane germplasm.  A.Vaze, G.Nerkar, M.Pagariya, R.M.Devarumath and D.Theertha Prasad Sugarcane, genomic DNA, PCR, RAPD, ISSR -