10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) The Gemcitabine loaded PLGA nanoparticles were prepared by modified nanoprecipitation method, to improve the bioavailability of the drug in brain. The nanoparticular formulation was characterized for particle size analysis, encapsulation efficiency, zeta potential and in vitro release study. The pharmacokinetic studies of Gemcitabine-loaded PLGA nanoparticles were carried out in Sprague Dawley rats. The study revealed C lessThan sub greaterThan max lessThan /sub greaterThan and T lessThan sub greaterThan max lessThan /sub greaterThan were significantly altered and clearance was less in brain and blood when compared to that of plain Gemcitabine solution. These results suggested that PLGA nanoparticles had considerably increased the transport of Gemcitabine across the blood brain barrier (BBB). B.V. NAGARJUNA YADAV AND S.SATHESH KUMAR Brain tumour, Gemcitabine, blood brain barrier (BBB), modified nanoprecipitation method, nanoparticles. 582-590