10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) lessThan i greaterThan Leucas aspera lessThan /i greaterThan linn. is commonly called as 'Pandharpheda' and is widely distributed throughout south India, having many medicinal properties. A sensitive, simple and accurate HPTLC method has been established for identification and quantification of beta-sitosterol from lessThan i greaterThan leucas aspera lessThan /i greaterThan linn. whole plant powder. Quantification was carried out on pre-coated plate of HPTLC silica gel 60 F lessThan sub greaterThan 254 lessThan /sub greaterThan using mobile phase (toluene: ethyl acetate: formic acid, 12:7:1,v/v/v). CAMAG scanner IV set at visible light (520nm) for detection and quantification. The percentage concentrations of the beta-sitosterol were found to be 0.0672.Response was linear over the range of 0.1µg to 0.5µg with 0.66 and 0.314 of %CV respectively.CAMAG TLC-MS interface was used for the confirmation of m/z values of beta-sitosterol in standard as well as sample. The m/z value was found to be 397(M+H lessThan sup greaterThan + lessThan /sup greaterThan -H lessThan sub greaterThan 2 lessThan /sub greaterThan O). The method i.e. HPTLC and TLC-MS together provides a rapid and secure method for detection, identification and quantification of biomarker beta-sitosterol. PRASHANT HANDE, B S AJITKUMAR, SHAILENDRA RANE,MANISH HATE, AND AKSHAY CHAREGAONKAR Leucas aspera linn., CAMAG HPTLC, CAMAG TLC-MS interface, Shimadzu LCMS-2020, Beta-sitosterol, Identification, Quantification. 628-636