10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) The objective of the present study was to evaluate the free radical scavenging activity and antioxidant potential of Canthium Coramandelium. Nitric oxide radical scavenging activity was determined using the method of Garrat (1964). Sodium nitroprusside in aqueous solution at physiological pH spontaneously generates nitric oxide which interacts with oxygen to produce nitrite ions which can be determined by the use of Griess Illosvoy reaction. Two milliliter of 10mM sodium nitroprusside in 0.5mL phosphate buffer saline (pH 7.4) was mixed with 0.5mL of canthium coromandelicum at various concentrations and the mixture incubated at 25*C for 150 min. From the incubated mixture 0.5mL was taken out and added into 1.0 mL sulfanilic acid reagent (30 in 20% glacial acetic acid) and incubated at room temperature for 5 min. Finally, 1.0mL naphthylethylenediamine dihydrochloride (0.1% w/v) was mixed and incubated at room temperature for 30min. The resultant absorbance was recorded at 540 nm using a spectrophotometer. The percentage inhibition was calculated. At concentrations of 20, 40, 60, 80 μg/ml percentage of inhibition observed was 11.32±0.20, 28.94±0.78, 65.28±0.27 and 92.09±0.25 respectively. Thus this invitro study suggest that Canthium Coramandelium possesses antioxidant properties. Prior phytochemical analysis Canthium Coramandelium shows the presence of flavonoids, tannins and glycosides (Mohan et.al). The antioxidant property of Canthium Coramandelium may be attributed to the presence of flavonoids and tannins. However pharmacokinetic and safety profile of Canthium Coramandelium requires pre-clinical testing prior to its clinical application in human beings. DR.V.P.KARTHIK AND DR.DARLING CHELLATHAI DAVID Canthium Coramandelium, Nitric Oxide, In-Vitro 695-700