10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) Most of the pathogenic bacterial strains have developed resistance against the commonly used antibacterial drugs. This problem is increasing at an alarming rate and has urged upon the need to search for novel antibacterial compounds from natural sources. Entomopathogenic fungi have proved to be very promising candidates as they are known to produce a vast array of bioactive compounds. Screening the antibacterial potential of such compounds has led to the discovery of efficiently active compounds in past researches. But the entomopathogenic flora needs to be explored more for such compounds. Therefore, the focus of this study was to determine the antibacterial activity of secondary metabolite of lessThan i greaterThan Beauveria bassiana lessThan /i greaterThan using the agar well diffusion method. It was found that secondary metabolite exhibited maximum antibacterial activity after 21 days of incubation. The activity was highest against lessThan i greaterThan Escherichia coli lessThan /i greaterThan MTCC 1679 and was good against lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan MTCC 6204 and lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan MTCC 441. But it was poor against lessThan i greaterThan Salmonella typhi lessThan /i greaterThan MTCC 733. The zones of inhibition obtained against them were 30mm, 24mm, 18mm and 6mm respectively. However no activity was expressed against lessThan i greaterThan Klebsiella pneumoniae lessThan /i greaterThan MTCC 4032 lessThan i greaterThan . lessThan /i greaterThan Thus results of this research work reveal that entomopathogenic fungal isolate lessThan i greaterThan Beauveria bassiana lessThan /i greaterThan could be used for production of broad spectrum and eco-friendly antibacterial compounds. SMRITI SEN, HARSHITA SHUKLA AND SARDUL SINGH SANDHU Pathogenic bacteria, entomopathogenic fungi, secondary metabolites, antibacterial activity. 51-58