10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) Main problem for lessThan i greaterThan Mycobacterium tuberculosis lessThan /i greaterThan growth is time. This study evaluates the performance of Bact/Alert 3D system for isolates and identification of mycobacteria. 100 clinical specimens from the chest and tuberculosis department were included. All were positive by Lowenstein Jensen (LJ) media in different time. All samples were also tested by Bact/ALERT 3D system for time management study. Samples were microscopically positive for Ziehl Neelsen staining. 25 negative controls were also studied. Maximum 76% samples showed positivity in 3 lessThan sup greaterThan rd lessThan /sup greaterThan week and Minimum 10% positivity reported in 5 lessThan sup greaterThan th lessThan /sup greaterThan week of incubation in BacT/ALERT 3D system. On the other hand only 31% positivity reported in 3 lessThan sup greaterThan rd lessThan /sup greaterThan week by LJ media. BacT/ALERT 3D system was suitable, less time consuming method over LJ media culture. It may be very useful not only to quick detection but also to provide faster treatment and a better prognosis. ASHISH K. ASTHANA AND MOLLY MADAN BacT/ALERT 3D system, Tuberculosis, Lowenstein Jensen media 844-847