10.22376/ijpbs.2019.10.1.p1-12 Volume 6 Issue 4 2015 (October - December) Chromium (Cr) is a well known toxic metal that affect the growth and development of plants. Assessment of genotypic variation for Cr tolerance is crucial to understand the insights of tolerance mechanism. A pot experiment was conducted in a green house to evaluate the genotypic variability in groundnut for Cr tolerance, with different concentrations of Cr. Thirteen groundnut genotypes were exposed to different levels of Cr and studied their relative responses in terms of growth and biochemical traits underlying across the genotypes. After one month of stress imposition, clearly differentiates the genotypes - in their growth parameters, viz., chlorophyll content, free proline, electrolyte leakage and antioxidative enzymes. The effect of Cr was severe with increasing concentration of Cr (100, 200, and 300 mg kg lessThan sup greaterThan −1 lessThan /sup greaterThan soil) on all genotypes studied. The shoot and root growth, biomass and chlorophyll content was severely affected at - higher concentration of Cr (300 kg lessThan sup greaterThan −1 lessThan /sup greaterThan soil). Free proline content and antioxidative enzymes like catalase (CAT) and Ascorbate peroxidase (APX) activity was significantly increased at (100 mg kg lessThan sup greaterThan −1 lessThan /sup greaterThan soil) Cr contamination, however the enzyme activities were gradually decreased at higher concentration of Cr (200, and 300 mg kg lessThan sup greaterThan −1 lessThan /sup greaterThan soil). The electrolyte leakage was also found to be increased with the increase in concentration of Cr. Based on -the data - genotypes studied can be categorized as - genotypes - highly tolerant (Abhaya, Anantha and Dharani )- as moderately tolerant (ICGV-91114, K-6, K-1375, TG-47 and Rohini) and sensitive (TPT-4, JL-24 and Narayahi) to Cr stress. JAYANNA NAIK BANAVATH, SRAVANI KONDURU, VARAKUMAR PANDIT1 , KRISHNA KUMAR GUDURU,SURESH RAJU K, SUDHAKAR PODHA, CHANDRA SEKHAR AKILA AND CHANDRA OBUL REDDY PULI1 Heavy metal stress, Chromium (Cr), Groundnut, Proline, Antioxidative Enzymes 1077-1089