International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 7 Issue 1
2016 (January - March)
EVALUATION OF ANTI-DIARRHOEAL ACTIVITY OF BACILLUS COAGULANS MTCC 5856 AND ITS EFFECT ON GASTROINTESTINAL MOTILITY IN WISTAR RATS
Probiotics are live microorganisms that confer a number of health beneï¬ts when consumed in adequate amounts. However, the beneficial effects of a probiotic are strain specific and hence each probiotic strain must be evaluated for their application. Thus, the present study was aimed to investigate the effect of lessThan i greaterThan Bacillus coagulans lessThan /i greaterThan MTCC 5856 on castor oil induced diarrhoea and gastrointestinal (GI) motility using well established rodent models. Rats were divided into 6 different groups with six rats in each group i.e., 1) normal control (no treatment), 2) positive control (loperamide, 1 mg/kg), 3) negative control (maltodextrin) and 4, 5 and 6 treatment groups receiving orally 40, 80 and 160 × 10 lessThan sup greaterThan 6 lessThan /sup greaterThan cfu/kg body weights of lessThan i greaterThan B. coagulans lessThan /i greaterThan MTCC 5856 spores respectively. Weight of faecal samples was recorded at 4, 8 and 12 h in castor oil induced diarrhoea. Furthermore, in an independent study, GI motility was assessed in fasting rats using charcoal meal as a marker. lessThan i greaterThan B. coagulans lessThan /i greaterThan MTCC 5856 showed dose dependent anti- diarrhoeal activity, and the percentage inhibition of faecal weight was similar as loperamide group at a dose of 160 × 10 lessThan sup greaterThan 6 lessThan /sup greaterThan cfu/kg body weights in 4 h (p greaterThan 0.05). However, the GI motility inhibition was not comparable with atropine sulphate (0.1 mg/kg body weight) which could be due to its intraperitoneal route of administration. lessThan i greaterThan B. coagulans lessThan /i greaterThan MTCC 5856 elicited anti-diarrhoeal activity and inhibited the gastrointestinal motility in fasted rats. Therefore, lessThan i greaterThan B. coagulans lessThan /i greaterThan MTCC 5856 could be a potential agent in the management of diarrhoea.
DR. MUHAMMED MAJEEDa,b, DR. S. NATARAJANa, DR. A. SIVAKUMARa, DR. FURQAN ALIa, DR. ANURAG PANDEb, MR. SHAHEEN MAJEEDc AND MR. SURESH KUMAR KARRId *
Bacillus coagulans, diarrhoea, intestinal motility, probiotic.
311-316