10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 3 2016 (July - September) One hundred and twenty three (123) blood samples from icteric patients, received for serum creatinine estimation were divided into different groups depending on bilirubin concentration -- Group I (1-5 mg/dl), Group II (5 -15 mg/dl), Group III (15-25 mg/dl), Group IV ( greaterThan 25 mg/dl). Twenty (20) blood samples from non-icteric patients within the normal reference range of bilirubin (0.2 - 0.8 mg/dl) were taken as controls, group V. All these samples were estimated for serum creatinine by four methods (1) Jaffe's kinetic method, (2) pre-incubation with NaOH method, (3) addition of SDS method and (4) enzymatic method (reference method). A significant increase in serum true creatinine was founded by pre-incubation with 200mmol/L NaOH and the addition of SDS method for bilirubin concentration greaterThan 15 mg/dl (p lessThan 0.01) and mild to moderate increase for bilirubin concentration 1-15 mg/dl. From this study, it was found that increased concentration of bilirubin interfered in creatinine value measurement except in Jaffe's kinetic method.  CHEEMALAVAGUPALLI REKHA, MANCHALA SRESHMITHA Glomerular Filtration Rate, Isotope Dilution Mass Spectrometry, Serum Bilirubin, Serum Creatinine, J 355-360