10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) Conjugated linoleic acids are the most biologically active fatty acids, synthesized by biohydrogenation of linoleic acid (C18:2).In the present study, lessThan i greaterThan Lactobacillus plantarum lessThan /i greaterThan was used as a probiotic model for the biotransformation of linoleic acid to conjugated linoleic acids. The therapeutic applications of conjugated linoleic acids were validated through in-vitro and in-vivo studies. The anti-inflammatory activity was evaluated with cotton pellet induced granuloma and hydroxyl proline content in wistar rats. Oral administration of conjugated linoleic acid and linoleic acid showed dose-dependent anti-inflammatory activity. Antioxidant propertiesof conjugated linoleic acid analyzed by catalase, superoxide and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay showed significant antioxidant activity. The anti-proliferative activity assessed through morphological findings and neutral red assay exhibited conjugated linoleic acid as an effective cytotoxic agent against Estrogen Receptor-negative breast cancer cell line. The results clearly endorse the traditional use of probiotics by confirming the therapeutic potential of conjugated linoleic acids. RASHMI HOLUR KADIDRAREDDY, VANI MATHAKALA AND UMA MAHESWARI DEVI PALEMPALLI Conjugated linoleic acids, Lactobacillus plantarum, linoleic acid, Neutral red assay 215-223