10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) In this study, the bioactive compounds present in the ethyl acetate extracts of two different lessThan i greaterThan Streptomyces lessThan /i greaterThan strains isolated from vermicast (earthworm fecal pellets) were investigated using Gas Chromatography and Mass Spectrometry (GC-MS). GC-MS analysis showed the presence of 12 and 9 bioactive compounds in AS1 and AS3 respectively. GC-MS analysis of AS1extract revealed the presence of major bioactive compounds such as n-hexadecanoic acid, 6-hexadecanoic acid, methyl ester, 9-octadeconic acid, methyl ester, 2-4, cycloheadien-1-one, 3,5-bis (1,1-dimethyl ethyl) 4-hydroxy and AS3 extract contains hexadecanoic acid, 9-octadecanic acid-E, oleic acid, 6-ocatadecanic acid, 2-4, cycloheadien-1-one, 3,5-bis (1,1-dimethyl ethyl) 4-hydroxy, 1,2-Benzisothiazol-3- amine tbdms, hexamethyl cyclotrisiloxane and 1,2-Bis (trimethylsilyl) benzene. R. BALACHANDAR, K. ASHOK KUMAR AND N. KARMEGAM GC-MS analysis, bioactive compounds, Streptomyces species, ethyl acetate extract, vermicast. 416-419