10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) In the present study it was aimed to improve the concentration of Menaquinone -7 (MK-7) by the potent strain KLSG-9 isolated from soil sample of Gulbarga region, India. Phylogenetic analysis of KLSG-9 strain was done by 16S rRNA partial sequencing and it revealed that isolate belongs to the genus lessThan i greaterThan Bacillus lessThan /i greaterThan . The highest similarity of 16S rRNA of KLSG-9 was shown with lessThan i greaterThan Bacillus thuringiensis lessThan /i greaterThan Bt407. The obtained sequence was submitted to GenBank under the accession number KT326332. Physical as well as nutritional parameters were optimized to obtain maximum MK-7 yield. The optimum pH and temperature for MK-7 production was 7 and 40°C whereas inoculum size was 2 ml, agitation speed 160 rpm with incubation period of 120h. Soya peptone, sucrose and glycerol were the best nitrogen and carbon sources, respectively, among the phosphate sources tested K lessThan sub greaterThan 2 lessThan /sub greaterThan HPO lessThan sub greaterThan 4 lessThan /sub greaterThan was found better source. In this regard with all optimum conditions we could achieve total 73 mg/L of MK-7 production. SANDHYA GAJARE, LINGAPPA KATTIMANI, SARFARAZ AHMED, MAHESH DIVATAR AND SHIVALEE A Menaquinone-7, MK-7, Vitamin K2, Osteoporosis and Bacillus thuringiensis. 420-426